Amazing Spider-Man (Vol. 1) Annual #23 (Story 5)
Background
Somebody at Marvel let Fred Hembeck do a series of small features on Spidey and his extraordinary history.
Story 'Spider-Man's Top 30 Villain Countdown'
Amazing Spider-Man (Vol. 1) Annual #23 (Story 5)
Here’s the breakdown of baddies along with their chief claim to fame:
30) The Red Skull – Killed and framed Spider-Man’s parents.
29) The Crime-Master – Masked wannabe mob leader.
28) The Sin Eater – Killed Captain Jean DeWolffe.
27) Hammerhead – Steel-headed wannabe mob leader.
26) The Chameleon – Spider-Man’s first costumed foe.
25) Hydro-Man – Like Sandman, only wetter.
24) The Shocker – Has a crude slang term named after him.
23) Molten Man – Liz Allen’s big brother.
22) Silvermane – Cyborg wannabe mob leader.
21) The Juggernaut – Unstoppable force of nature.
20) The Human Fly – A spider’s natural prey.
19) Carrion – Clone of Peter’s college professor.
18) The Jackal – Peter’s college professor, father of the clone saga.
17) The Tarantula – Evil opposite, kinda.
16) The Rhino – Semi-unstoppable force of nature.
15) Puma – Feline mercenary.
14) The Lizard – Followed by Stegron, the Iguana, etc.
13) Electro – Like the Energizer Bunny, only evil.
12) Mysterio – Surprisingly agile for a man with a fish-bowl on his head.
11) Sandman – Is he a good guy or a bad guy?
10) J.J.J. – ‘Nuff said.
9) The Burglar – The catalyst for all that followed.
8) Venom – Alien symbiote gone mad, duh.
7) The Scorpion – A spider’s natural predator.
6) The Vulture – Surprisingly nimble for a septuagenarian.
5) Dr. Doom – On loan from the Fantastic Four.
4) The Kingpin – On loan from Daredevil.
3) Kraven – Recently deceased.
2) Dr. Octopus – Of course.
1) Green Goblin & Hobgoblin – Was there any doubt?
General Comments
I love these types of lists. It’s been 25 years since this was published. I would love to see one of this done for the modern age just to compare the differences.
Overall Rating 
The only thing that’s missing is issue annotations for all of these characters. But what do you expect from a 3-page feature?